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Improved Diagnosis for Nine Viral Diseases Considered as Notifiable By the World Organization for Animal Health

Identifieur interne : 000C53 ( Istex/Curation ); précédent : 000C52; suivant : 000C54

Improved Diagnosis for Nine Viral Diseases Considered as Notifiable By the World Organization for Animal Health

Auteurs : B. Rodriguez-Sanchez [Espagne] ; J. M. Sanchez-Vizcaino [Espagne] ; Uttenthal [Danemark] ; T. B. Rasmussen [Danemark] ; M. Hakhverdyan [Suède] ; D. P. King [Royaume-Uni] ; N. P. Ferris [Royaume-Uni] ; K. Ebert [Royaume-Uni] ; S. M. Reid [Royaume-Uni] ; I. Kiss [Hongrie] ; E. Brocchi [Italie] ; P. Cordioli [Italie] ; B. Hjerner [Royaume-Uni] ; M. Mcmenamy [Royaume-Uni] ; J. Mckillen [Royaume-Uni] ; J. S. Ahmed [Allemagne] ; S. Belak [Suède]

Source :

RBID : ISTEX:6F30A742A672BE8A10885D893591C9A8CC00DA40

Abstract

Nine viral diseases included in the World Organization for Animal Health list of notifiable diseases (former list A) were chosen for their contagiousness and high capacity of spreading to improve their diagnosis using new and emerging technologies. All the selected diseases – foot‐and‐mouth disease, swine vesicular disease, vesicular stomatitis, classical swine fever, African swine fever, bluetongue, African horse sickness, Newcastle disease and highly pathogenic avian influenza – are considered as transboundary diseases, which detection causes the prohibition of livestock exportation, and, thus, it leads to high economical losses. The applied diagnostic techniques can fall into two categories: (i) nucleic‐acid detection, including padlock probes, real‐time PCR with TaqMan, minor groove binding probes and fluorescence energy transfer reaction probes, isothermal amplification like the Cleavase/Invader assay or the loop‐mediated amplification technology and the development of rapid kits for ‘mobile’ PCR and (ii) antigen‐antibody detection systems like simplified and more sensitive ELISA tests. Besides, internal controls have been improved for nucleic acid‐detecting methods by using an RNA plant virus – Cowpea Mosaic Virus – to ensure the stability of the RNA used as a positive control in diagnostic real‐time RT‐PCR assays. The development of these diagnosis techniques has required the joint efforts of a European consortium in which nine diagnostic laboratories and an SME who have collaborated since 2004 within the European Union‐funded Lab‐on‐site project. The results obtained are shown in this paper.

Url:
DOI: 10.1111/j.1865-1682.2008.01037.x

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ISTEX:6F30A742A672BE8A10885D893591C9A8CC00DA40

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<title level="j" type="main">Transboundary and Emerging Diseases</title>
<title level="j" type="alt">TRANSBOUNDARY AND EMERGING DISEASES</title>
<idno type="ISSN">1865-1674</idno>
<idno type="eISSN">1865-1682</idno>
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<div type="abstract" xml:lang="en">Nine viral diseases included in the World Organization for Animal Health list of notifiable diseases (former list A) were chosen for their contagiousness and high capacity of spreading to improve their diagnosis using new and emerging technologies. All the selected diseases – foot‐and‐mouth disease, swine vesicular disease, vesicular stomatitis, classical swine fever, African swine fever, bluetongue, African horse sickness, Newcastle disease and highly pathogenic avian influenza – are considered as transboundary diseases, which detection causes the prohibition of livestock exportation, and, thus, it leads to high economical losses. The applied diagnostic techniques can fall into two categories: (i) nucleic‐acid detection, including padlock probes, real‐time PCR with TaqMan, minor groove binding probes and fluorescence energy transfer reaction probes, isothermal amplification like the Cleavase/Invader assay or the loop‐mediated amplification technology and the development of rapid kits for ‘mobile’ PCR and (ii) antigen‐antibody detection systems like simplified and more sensitive ELISA tests. Besides, internal controls have been improved for nucleic acid‐detecting methods by using an RNA plant virus – Cowpea Mosaic Virus – to ensure the stability of the RNA used as a positive control in diagnostic real‐time RT‐PCR assays. The development of these diagnosis techniques has required the joint efforts of a European consortium in which nine diagnostic laboratories and an SME who have collaborated since 2004 within the European Union‐funded Lab‐on‐site project. The results obtained are shown in this paper.</div>
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